Disruption of the standard kinetochore in holocentric Cuscuta species
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00584248" target="_blank" >RIV/60077344:_____/23:00584248 - isvavai.cz</a>
Result on the web
<a href="https://www.pnas.org/doi/10.1073/pnas.2300877120" target="_blank" >https://www.pnas.org/doi/10.1073/pnas.2300877120</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.2300877120" target="_blank" >10.1073/pnas.2300877120</a>
Alternative languages
Result language
angličtina
Original language name
Disruption of the standard kinetochore in holocentric Cuscuta species
Original language description
The segregation of chromosomes depends on the centromere. Most species are monocentric, with the centromere restricted to a single region per chromosome. In some organisms, the monocentric organization changed to holocentric, in which the centromere activity is distributed over the entire chromosome length. However, the causes and consequences of this transition are poorly understood. Here, we show that the transition in the genus Cuscuta was associated with dramatic changes in the kinetochore, a protein complex that mediates the attachment of chromosomes to microtubules. We found that in holocentric Cuscuta species, the KNL2 genes were lost, the CENP-C, KNL1, and ZWINT1 genes were truncated, the centromeric localization of CENH3, CENP-C, KNL1, MIS12, and NDC80 proteins was disrupted, and the spindle assembly checkpoint (SAC) degenerated. Our results demonstrate that holocentric Cuscuta species lost the ability to form a standard kinetochore and do not employ SAC to control the attachment of microtubules to chromosomes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
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Volume of the periodical
120
Issue of the periodical within the volume
21
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
e2300877120
UT code for WoS article
001039496800005
EID of the result in the Scopus database
2-s2.0-85161349225