Dominant suppressor genes of p53-induced apoptosis in Drosophila melanogaster.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00588413" target="_blank" >RIV/60077344:_____/24:00588413 - isvavai.cz</a>

Result on the web

<a href="https://academic.oup.com/g3journal/article-pdf/14/9/jkae149/59020940/jkae149.pdf" target="_blank" >https://academic.oup.com/g3journal/article-pdf/14/9/jkae149/59020940/jkae149.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/g3journal/jkae149" target="_blank" >10.1093/g3journal/jkae149</a>

Result language

angličtina

Original language name

Dominant suppressor genes of p53-induced apoptosis in Drosophila melanogaster.

Original language description

One of a major function of programmed cell death (apoptosis) is the removal of cells which suffered oncogenic mutations, thereby preventing cancerous transformation. By making use of a Double-Headed-EP (DEP) transposon, a P element derivative made in our laboratory, we made an insertional mutagenesis screen in Drosophila melanogaster to identify genes which, when overexpressed, suppress the p53-activated apoptosis. The DEP element has Gal4-activatable, outward-directed UAS-promoters at both ends which can be deleted separately in vivo. In the DEP insertion mutants, we used the GMR-Gal4 driver to induce transcription from both UAS-promoters and tested the suppression effect on the apoptotic rough eye phenotype generated by an activated UAS-p53 transgene. By DEP insertions, seven genes were identified which suppressed the p53-induced apoptosis. In four mutants, the suppression effect resulted from single genes activated by one UAS-promoter (Pka-R2, Rga, crol, Spt5). In the other three (Orct2, Polr2M, stg), deleting either UAS-promoter eliminated the suppression effect. In qPCR experiments we found that the genes in the vicinity of the DEP insertion also showed an elevated expression level. This suggested an additive effect of the nearby genes on suppressing apoptosis. In the eucaryotic genomes there are co-expressed gene clusters. Three of the DEP insertion mutants are included and two are in close vicinity of separate co-expressed gene clusters. This raises the possibility that the activity of some of the genes in these clusters may help the suppression of the apoptotic cell death.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

G3-Genes, Genomes, Genetics

ISSN

2160-1836

e-ISSN

2160-1836

Volume of the periodical

14

Issue of the periodical within the volume

9

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

jkae149

UT code for WoS article

001281452900001

EID of the result in the Scopus database

2-s2.0-85203418753