Fatty acids and their derivatives from Chlorella vulgaris extracts exhibit in vitro antimicrobial activity against the honey bee pathogen Paenibacillus larvae

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00599744" target="_blank" >RIV/60077344:_____/24:00599744 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/24:00599744 RIV/61989592:15310/24:73616791

Result on the web

<a href="https://www.tandfonline.com/doi/full/10.1080/00218839.2021.1994264?scroll=top&needAccess=true" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/00218839.2021.1994264?scroll=top&needAccess=true</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/00218839.2021.1994264" target="_blank" >10.1080/00218839.2021.1994264</a>

Result language

angličtina

Original language name

Fatty acids and their derivatives from Chlorella vulgaris extracts exhibit in vitro antimicrobial activity against the honey bee pathogen Paenibacillus larvae

Original language description

The green microalga Chlorella vulgaris Beijerinck (Chlorellaceae) is widely used as a food supplement for humans and animals. In beekeeping practice, Chlorella vulgaris has potential as a pollen supplement. We studied whether Chlorella extracts display antimicrobial properties against Paenibacillus larvae, the causative agent of the honey bee bacterial disease American foulbrood. We identified components responsible for antimicrobial activity and evaluated the added values of Chlorella as a food supplement for honey bees. Different extracts (water, acetone, methanol) were prepared from Chlorella biomass (phototrophically and heterotrophically cultivated) and screened for antimicrobial activity against ERIC I and ERIC II genotypes of P. larvae. Active acetone extracts of phototrophically cultivated Chlorella vulgaris biomass were fractioned via preparative reverse-phase chromatography. Antimicrobial activity was detected for 9 of the resulting 33 fractions. Further analysis revealed the chemical composition of the active fractions. C. vulgaris extracts showed a significant antimicrobial effect against vegetative cells and spores of P. larvae strains of ERIC I and ERIC II genotypes. The lowest MIC of the most active acetone extract was 6.3 mu g/mL for both tested genotypes. In the majority of the active fractions, monolinolenin, fatty acid linoleic acid, and methyl esters of linoleic and/or palmitic acid were identified via high-performance liquid chromatography coupled with high-resolution mass spectrometry analysis. Based on our results, we concluded that algal C. vulgaris food supplements not only contain nutritional but also potential prophylactic properties for honey bee health.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10616 - Entomology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Apicultural Research

ISSN

0021-8839

e-ISSN

2078-6913

Volume of the periodical

63

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

310-322

UT code for WoS article

000716298500001

EID of the result in the Scopus database

2-s2.0-85118626218