CRISPR-mediated iron and folate biofortification in crops: advances and perspectives

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00603871" target="_blank" >RIV/60077344:_____/24:00603871 - isvavai.cz</a>

Result on the web

<a href="https://www.tandfonline.com/doi/full/10.1080/02648725.2023.2205202" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/02648725.2023.2205202</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/02648725.2023.2205202" target="_blank" >10.1080/02648725.2023.2205202</a>

Result language

angličtina

Original language name

CRISPR-mediated iron and folate biofortification in crops: advances and perspectives

Original language description

Micronutrient deficiency conditions, such as anemia, are the most prevalent global health problem due to inadequate iron and folate in dietary sources. Biofortification advancements can propel the rapid amelioration of nutritionally beneficial components in crops that are required to combat the adverse effects of micronutrient deficiencies on human health. To date, several strategies have been proposed to increase micronutrients in plants to improve food quality, but very few approaches have intrigued clustered regularly interspaced short palindromic repeats (CRISPR) modules for the enhancement of iron and folate concentration in the edible parts of plants. In this review, we discuss two important approaches to simultaneously enhance the bioavailability of iron and folate concentrations in rice endosperms by utilizing advanced CRISPR-Cas9-based technology. This includes the tuning of cis-elements and enhancer re-shuffling in the regulatory components of genes that play a vital role in iron and folate biosynthesis/transportation pathways. In particular, base-editing and enhancer re-installation in native promoters of selected genes can lead to enhanced accumulation of iron and folate levels in the rice endosperm. The re-distribution of micronutrients in specific plant organs can be made possible using the above-mentioned contemporary approaches. Overall, the present review discusses the possible approaches for synchronized iron and folate biofortification through modification in regulatory gene circuits employing CRISPR-Cas9 technology.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biotechnology & Genetic Engineering Reviews

ISSN

0264-8725

e-ISSN

2046-5556

Volume of the periodical

40

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

27

Pages from-to

4138-4168

UT code for WoS article

000974987100001

EID of the result in the Scopus database

2-s2.0-85153528947