Tracing de novo lipids using stable isotope labeling LCTIMS-TOF MS/MS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00604072" target="_blank" >RIV/60077344:_____/24:00604072 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.3791/65590" target="_blank" >https://doi.org/10.3791/65590</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3791/65590" target="_blank" >10.3791/65590</a>
Alternative languages
Result language
angličtina
Original language name
Tracing de novo lipids using stable isotope labeling LCTIMS-TOF MS/MS
Original language description
Lipids are highly diverse, and small changes in lipid structures and composition can have profound effects on critical biological functions. Stable isotope labeling (SIL) offers several advantages for the study of lipid distribution, mobilization, and metabolism, as well as de novo lipid synthesis. The successful implementation of the SIL technique requires the removal of interferences from endogenous molecules. In the present work, we describe a high-throughput analytical protocol for the screening of SIL lipids from biological samples examples will be shown of lipid de novo identification during mosquito ovary development. The use of complementary liquid chromatography trapped ion mobility spectrometry and mass spectrometry allows for the separation and lipids assignment from a single sample in a single scan (<1 h). The described approach takes advantage of recent developments in data-dependent acquisition and data-independent acquisition, using parallel accumulation in the mobility trap followed by sequential fragmentation and collision-induced dissociation. The measurement of SIL at the fatty acid chain level reveals changes in lipid dynamics during the ovary development of mosquitoes. The lipids de novo structures are confidently assigned based on their retention time, mobility, and fragmentation pattern.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA22-21244S" target="_blank" >GA22-21244S: Deciphering juvenile hormone (JH) dependent regulation of development and reproduction in mosquitoes using JH null mutants</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Jove-Journal of Visualized Experiments
ISSN
1940-087X
e-ISSN
1940-087X
Volume of the periodical
AUG
Issue of the periodical within the volume
210
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
e65590
UT code for WoS article
001322735800002
EID of the result in the Scopus database
2-s2.0-85203784557