Diversity of mycorrhizal fungi in temperate orchid species: Comparison of culture-dependent and culture-independent methods

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00605732" target="_blank" >RIV/60077344:_____/24:00605732 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/24:43908342

Result on the web

<a href="https://doi.org/10.3390/jof10020092" target="_blank" >https://doi.org/10.3390/jof10020092</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/jof10020092" target="_blank" >10.3390/jof10020092</a>

Result language

angličtina

Original language name

Diversity of mycorrhizal fungi in temperate orchid species: Comparison of culture-dependent and culture-independent methods

Original language description

Many orchid species are endangered due to anthropogenic pressures such as habitat destruction and overharvesting, meanwhile, all orchids rely on orchid mycorrhizal fungi (OMF) for seed germination and seedling growth. Therefore, a better understanding of this intimate association is crucial for orchid conservation. Isolation and identification of OMF remain challenging as many fungi are unculturable. In our study, we tested the efficiency of both culture-dependent and culture-independent methods to describe OMF diversity in multiple temperate orchids and assessed any phylogenetic patterns in cultivability. The culture-dependent method involved the cultivation and identification of single pelotons (intracellular hyphal coils), while the culture-independent method used next-generation sequencing (NGS) to identify root-associated fungal communities. We found that most orchid species were associated with multiple fungi, and the orchid host had a greater impact than locality on the variability in fungal communities. The culture-independent method revealed greater fungal diversity than the culture-dependent one, but despite the lower detection, the isolated fungal strains were the most abundant OMF in adult roots. Additionally, the abundance of NGS reads of cultured OTUs was correlated with the extent of mycorrhizal root colonization in orchid plants. Finally, this limited-scale study tentatively suggests that the cultivability character of OMF may be randomly distributed along the phylogenetic trees of the rhizoctonian families.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Fungi

ISSN

2309-608X

e-ISSN

2309-608X

Volume of the periodical

10

Issue of the periodical within the volume

2

Country of publishing house

CH - SWITZERLAND

Number of pages

21

Pages from-to

92

UT code for WoS article

001172109400001

EID of the result in the Scopus database

2-s2.0-85187252000