Short- and mid-term temporal variability of the human urinary microbiota: a prospective observational cohort study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00636159" target="_blank" >RIV/60077344:_____/25:00636159 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/25:00636159 RIV/00216208:11120/25:43928375 RIV/00064211:_____/25:W0000041

Result on the web

<a href="https://bmcmicrobiol.biomedcentral.com/counter/pdf/10.1186/s12866-025-03915-7.pdf" target="_blank" >https://bmcmicrobiol.biomedcentral.com/counter/pdf/10.1186/s12866-025-03915-7.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12866-025-03915-7" target="_blank" >10.1186/s12866-025-03915-7</a>

Result language

angličtina

Original language name

Short- and mid-term temporal variability of the human urinary microbiota: a prospective observational cohort study

Original language description

Background: Understanding the temporal variability of the microbiome is critical for translating associations of the microbiome with health and disease into clinical practice. The aim of this study is to assess the extent of temporal variability of the human urinary microbiota. A pair of urine samples were collected from study participants at 3-40-month interval. DNA was extracted and the bacterial V4 hypervariable region of the 16S rRNA gene was sequenced on the Illumina MiSeq platform. The alpha diversity of paired samples was analyzed using Chao1 and Shannon indices and PERMANOVA was used to test the factors influencing beta diversity. Results: A total of 63 participants (43 men and 20 women with a mean age of 63.0 and 57.1 years, respectively) were included in the final analysis. An average of 152 ± 128 bacterial operational taxonomic units (OTUs) were identified in each urine sample from the entire cohort. There was an average of 41 ± 32 overlapping OTUs in each sample pair, accounting for 66.3 ± 29.4% of the relative abundance. There was a clear correlation between the number of overlapping OTUs and the relative abundance covered. The difference in Chao1 index between paired samples was statistically significant, the difference in Shannon index was not. Beta diversity did not differ significantly within the paired samples. Neither age nor sex of the participants influenced the variation in community composition. With a longer interval between the collections, the relative abundance covered by the overlapping OTUs changed significantly but not the number of OTUs. Conclusion: Our findings demonstrated that, while the relative abundance of dominant bacteria varied, repeated collections generally shared more than 60% of the bacterial community. Furthermore, we observed little variation in the alpha and beta diversity of the microbial community in human urine. These results help to understand the dynamics of human urinary microbiota and enable interpretation of future studies.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GN23-07434O" target="_blank" >GN23-07434O: TIMPAOMP project - The IMPortance of Anaerobic Oxidation of Methane in Peatlands</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Microbiology

ISSN

1471-2180

e-ISSN

1471-2180

Volume of the periodical

25

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

222

UT code for WoS article

001469271500002

EID of the result in the Scopus database

2-s2.0-105002995429