Converting Blastocrithidia Nonstop, a Trypanosomatid With Non-Canonical Genetic Code, Into a Genetically-Tractable Model
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00636806" target="_blank" >RIV/60077344:_____/25:00636806 - isvavai.cz</a>
Alternative codes found
RIV/61988987:17310/25:A2603CPB RIV/60076658:12310/25:43909898 RIV/00216208:11310/25:10515621
Result on the web
<a href="https://doi.org/10.1111/mmi.15365" target="_blank" >https://doi.org/10.1111/mmi.15365</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/mmi.15365" target="_blank" >10.1111/mmi.15365</a>
Alternative languages
Result language
angličtina
Original language name
Converting Blastocrithidia Nonstop, a Trypanosomatid With Non-Canonical Genetic Code, Into a Genetically-Tractable Model
Original language description
Blastocrithidia nonstop is a protist with a highly unusual nuclear genetic code, in which all three standard stop codons are reassigned to encode amino acids, with UAA also serving as a sole termination codon. In this study, we demonstrate that this parasitic flagellate is amenable to genetic manipulation, enabling gene ablation and protein tagging. Using preassembled Cas9 ribonucleoprotein complexes, we successfully disrupted and tagged the non-essential gene encoding catalase. These advances establish this single-celled eukaryote as a model organism for investigating the malleability and evolution of the genetic code in eukaryotes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Microbiology
ISSN
0950-382X
e-ISSN
1365-2958
Volume of the periodical
123
Issue of the periodical within the volume
6
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
586-592
UT code for WoS article
001462570000001
EID of the result in the Scopus database
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