Key RNA-binding domains in the La protein establish tRNA modification levels in Trypanosoma brucei
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00637548" target="_blank" >RIV/60077344:_____/25:00637548 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/25:43910296
Result on the web
<a href="https://doi.org/10.1093/nar/gkaf594" target="_blank" >https://doi.org/10.1093/nar/gkaf594</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gkaf594" target="_blank" >10.1093/nar/gkaf594</a>
Alternative languages
Result language
angličtina
Original language name
Key RNA-binding domains in the La protein establish tRNA modification levels in Trypanosoma brucei
Original language description
The RNA-binding protein La is found in most eukaryotes, and despite being essential in many organisms, its function is not completely clear. Trypanosoma brucei, the causative agent of human African trypanosomiasis, encodes a 'classical' La protein (TbLa) composed of a La-motif, two RNA recognition motifs (RRM1 and RRM2 alpha), a C-terminal short basic motif (SBM), and a nuclear localization signal (NLS). In T. brucei, like in most eukaryotes, position 34 of tRNATyr,Asp,Asn andHis is modified with queuosine (Q34). The steady-state levels of queuosine-modified tRNA in the insect form (procyclic) of T. brucei can fluctuate dynamically depending on growth conditions, but the mechanism(s) controlling Q34 levels are not well understood. A well-established function of La is in precursor-tRNA 3 '-end metabolism, but in this work, we demonstrate that La also controls Q34-tRNA levels. Individual domain deletions showed that while deletion of La motif or RRM1 causes dysregulation of Q34-tRNA levels, no other domain plays a similar role. We also show that La is important for the normal balance of several additional tRNA modifications. These findings are discussed in the context of substrate competition between La and modification enzymes, also highlighting subcellular localization as a key determinant of tRNA function.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nucleic Acids Research
ISSN
0305-1048
e-ISSN
1362-4962
Volume of the periodical
53
Issue of the periodical within the volume
13
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
gkaf594
UT code for WoS article
001525153800001
EID of the result in the Scopus database
2-s2.0-105010623488