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Hidden Plasmodium diversity revealed in southeastern Asian passerines using next-generation amplicon sequencing

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00639443" target="_blank" >RIV/60077344:_____/25:00639443 - isvavai.cz</a>

  • Alternative codes found

    RIV/60076658:12310/25:43909849

  • Result on the web

    <a href="https://doi.org/10.1016/j.crpvbd.2025.100319" target="_blank" >https://doi.org/10.1016/j.crpvbd.2025.100319</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.crpvbd.2025.100319" target="_blank" >10.1016/j.crpvbd.2025.100319</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Hidden Plasmodium diversity revealed in southeastern Asian passerines using next-generation amplicon sequencing

  • Original language description

    Monitoring haemosporidian (Genus Plasmodium) infections in passerine birds is essential for understanding the intricate dynamics of avian malaria and its implications for ecology and evolution of avian populations. In this study, we investigated the prevalence and diversity of malaria infections caused by Plasmodium species in three passerine species in Cat-Tien National Park, Vietnam. Using next-generation amplicon sequencing (NGS) of haemosporidian cytochrome b gene, we identified two known and ten novel Plasmodium lineages. Our genetic analysis revealed a high rate of Plasmodium infections in the little spiderhunter (Arachnothera longirostra, Nectariniidae), the white-rumped shama (Copsychus malabaricus, Muscicapidae) and the blue-winged pitta (Pitta moluccensis, Pittidae). Species delimitation methods identified five distinct operational taxonomic units (OTUs), consistently across the majority of the used methods. Each of the passerine species was infected with a specific subset of the total Plasmodium diversity. Phylogenetic analysis showed that sympatric Plasmodium OTUs are not closely related and possess overlapping host preferences. Our findings may reflect differences in habitat use, such as the vertical strata occupied by different bird species, which contribute to varying exposure levels to suitable vectors, thereby influencing infection rates and parasite diversity. Our findings corroborate the view that avian malaria parasites are not uniformly opportunistic, rather, their distribution is filtered by host identity and ecology. Understanding these dynamics is crucial for avian conservation and broader ecological studies, as avian malaria serves as a model for studying parasite-vector-host co-evolution and the impact of environmental changes on disease dynamics.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30310 - Parasitology

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2025

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Current Research in Parasitology and Vector-Borne Diseases

  • ISSN

    2667-114X

  • e-ISSN

    2667-114X

  • Volume of the periodical

    8

  • Issue of the periodical within the volume

    SEP 2025

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    12

  • Pages from-to

    100319

  • UT code for WoS article

    001577756900001

  • EID of the result in the Scopus database

    2-s2.0-105016474246