Cryptosporidium geckonae n. sp. (Apicomplexa: Cryptosporidiidae) in geckos

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00640498" target="_blank" >RIV/60077344:_____/25:00640498 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12220/25:43910396

Result on the web

<a href="https://doi.org/10.1186/s13071-025-07068-4" target="_blank" >https://doi.org/10.1186/s13071-025-07068-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13071-025-07068-4" target="_blank" >10.1186/s13071-025-07068-4</a>

Result language

angličtina

Original language name

Cryptosporidium geckonae n. sp. (Apicomplexa: Cryptosporidiidae) in geckos

Original language description

BackgroundCryptosporidium is a globally prevalent parasite that infects the gastrointestinal tract of a large number of vertebrates. The Cryptosporidium sp. lizard genotype is found almost exclusively in geckos but has also been detected in a corn snake. In this study, the biology and genetic variability of the lizard genotype were investigated.MethodsThe genetic variability of the Cryptosporidium sp. lizard genotype was analysed by polymerase chain reaction (PCR) and Sanger sequencing of the SSU rRNA, actin, HSP70 and COWP genes. Its biological characteristics, including oocyst size, tissue tropism and associated pathology, were investigated using light microscopy, electron microscopy and histological techniques. Experimental infections were performed to assess host specificity and infectivity.ResultsPhylogenetic analysis of the target genes confirmed that the Cryptosporidium sp. lizard genotype is genetically distinct from other Cryptosporidium species. Experimental infections showed that chickens, mice and corn snakes were not susceptible to this genotype, as no infection was detected. In contrast, leopard geckos began to excrete oocysts between 6 and 8 days post-infection, although they showed no clinical symptoms. Excretion of oocysts continued for over 200 days. Oocysts isolated from naturally infected leopard and smooth knob-tailed geckos were morphometrically identical to those from experimentally infected leopard geckos and measured 5.89 x 4.79 mu m. The parasite mainly colonised the stomach, where the highest intensity of infection was observed, but was also found throughout the intestine and in the lungs.ConclusionsDue to the distinct genetic differences, host range and tissue tropism, we propose the name Cryptosporidium geckonae n. sp. for this organism, which was previously referred to as Cryptosporidium sp. lizard genotype.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Parasites & Vectors

ISSN

1756-3305

e-ISSN

1756-3305

Volume of the periodical

18

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

16

Pages from-to

420

UT code for WoS article

001597433600005

EID of the result in the Scopus database

2-s2.0-105019743992