Kinetoplast DNA structure, RNA editing patterns and small respiratory Complex I in Trypanosoma musculi
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00641685" target="_blank" >RIV/60077344:_____/25:00641685 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/25:43910335
Result on the web
<a href="https://doi.org/10.1038/s42003-025-08883-2" target="_blank" >https://doi.org/10.1038/s42003-025-08883-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s42003-025-08883-2" target="_blank" >10.1038/s42003-025-08883-2</a>
Alternative languages
Result language
angličtina
Original language name
Kinetoplast DNA structure, RNA editing patterns and small respiratory Complex I in Trypanosoma musculi
Original language description
Kinetoplast (k) DNA, a specialized form of mitochondrial DNA in trypanosomes, is composed of catenated maxicircles and minicircles, and is essential for the survival of these parasitic protists. In this study, we provide a detailed description of the near-complete minicircle complement of Trypanosoma musculi, a globally distributed, mouse-specific haemoflagellate. We have identified 39 minicircle classes, which can be grouped via their structural organization into five distinct categories. Using RNA-seq, we have reconstructed mitochondrial transcripts of ATP6, RPS12, CYTB, COX2 and COX3, all of which undergo canonical editing that renders them translatable, as well as abnormally edited transcripts of four respiratory Complex I subunits. Additionally, through a combined analysis of the kDNA minicircles, edited mRNAs and small RNAs, we identified 108 guide (g)RNA genes and further categorized their conservation in comparison with Trypanosoma lewisi, an opportunistic human parasite. Moreover, we demonstrate a small respiratory Complex I in T. musculi, highlighting the functional consequences of missing gRNAs. Finally, we propose a model for the evolutionary maintenance of gRNAs, contributing to our understanding of kDNA dynamics in trypanosomes and other kinetoplastid flagellates.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Communications Biology
ISSN
2399-3642
e-ISSN
2399-3642
Volume of the periodical
8
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
1544
UT code for WoS article
001610138200003
EID of the result in the Scopus database
2-s2.0-105021068033