The mitochondrial proteome of diplonemids: from conventional pathways to eccentric RNA editing and transcript processing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00643792" target="_blank" >RIV/60077344:_____/25:00643792 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/25:43910747

Result on the web

<a href="https://doi.org/10.1186/s12864-025-12233-1" target="_blank" >https://doi.org/10.1186/s12864-025-12233-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12864-025-12233-1" target="_blank" >10.1186/s12864-025-12233-1</a>

Result language

angličtina

Original language name

The mitochondrial proteome of diplonemids: from conventional pathways to eccentric RNA editing and transcript processing

Original language description

Background Diplonemids constitute an abundant and geographically widespread but little-studied group of marine protists. A hallmark of this lineage, the kinetoplastid sister group within Euglenozoa, is a mitochondrial genome comprising numerous small circular DNA molecules that carry fragments of mitochondrial genes. Complex RNA processing of the corresponding transcripts involves numerous ligation and RNA editing steps in the production of mature RNA species.To assess the diplonemid mitochondrial proteome and, in particular, to search for proteins that might mediate RNA processing, we undertook a comprehensive in silico analysis to predict candidate mitochondrial proteins in the type species Diplonema papillatum. Results Using sequence similarity searches in conjunction with a mitochondrial targeting pipeline, we identified at least 1878 candidate nucleus-encoded mitochondrial proteins in addition to 16 mitochondrion-encoded proteins described previously. Despite the highly unconventional nature of the mitochondrial genome in D. papillatum, its mitochondrial proteome (mitoproteome) contains virtually all the functionally most important proteins that are ubiquitous among aerobic mitochondria, and several novel proteins that have been recruited in the euglenozoan last common ancestor to augment complexes involved in coupled electron transport oxidative phosphorylation and mitochondrial ribosome formation. Notably, we identified several individual proteins and multi-protein families that are candidates for RNA ligation and editing enzymes. Conclusions This first comprehensive mitoproteome data for a diplonemid, together with published mitoproteome data for other members of Discoba, allows us to make inferences about marked changes in mitochondrial structure and function that have occurred since the divergence of diplonemids and other euglenozoans from the last common discobid ancestor.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

<a href="/en/project/GX23-06479X" target="_blank" >GX23-06479X: What do the most abundant oceanic protistan proteins actually do?</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Genomics

ISSN

1471-2164

e-ISSN

1471-2164

Volume of the periodical

26

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

27

Pages from-to

1099

UT code for WoS article

001637728600001

EID of the result in the Scopus database

2-s2.0-105024745354