Sampling strategy matters: eDNA-based assessment and extrapolation of myxozoan diversity in a model stream system
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00644767" target="_blank" >RIV/60077344:_____/25:00644767 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/25:43910543
Result on the web
<a href="https://doi.org/10.1002/oik.11282" target="_blank" >https://doi.org/10.1002/oik.11282</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/oik.11282" target="_blank" >10.1002/oik.11282</a>
Alternative languages
Result language
angličtina
Original language name
Sampling strategy matters: eDNA-based assessment and extrapolation of myxozoan diversity in a model stream system
Original language description
Determining true species diversity is particularly challenging for parasitic organisms. Environmental DNA (eDNA) metabarcoding has revolutionized diversity studies by increasing both efficiency and precision. This approach is especially relevant for parasitic species, like myxozoans that spend part of their life cycle outside their hosts, often during host-switching stages. However, the adequacy of sampling depth and methodology in capturing total diversity often remains uncertain. To address this, we used a small stream as a model system and collected 60 small-volume aquatic sediment samples. Using an eDNA metabarcoding approach with myxozoan-specific primers, including newly designed primers for Malacosporea and the Paramyxidium group, we identified 30 myxozoan OTUs. These were predominantly from the Myxobolus clade but represented a broad spectrum of freshwater myxozoans, including Malacosporea. Rarefaction and extrapolation analyses estimated the potential maximum myxozoan diversity at 40 OTUs, 25% higher than the observed diversity. Although approximately a quarter of OTUs were probably missed, our analysis suggested that additional sampling would not be efficient, as most newly detected OTUs would overlap with those already identified or belong to very rare species. We demonstrated that eDNA-based methods reliably detect myxozoan diversity in small sediment volumes and that statistical approaches can effectively estimate true diversity and assess the need for further sampling at a given locality.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10618 - Ecology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Oikos
ISSN
0030-1299
e-ISSN
1600-0706
Volume of the periodical
2025
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
e11282
UT code for WoS article
001556404500001
EID of the result in the Scopus database
2-s2.0-105013550773