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Statistical correlations of primer thermodynamic stability delta G0 for enhanced flax ISSR-PCR cultivar authentication

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077352%3A_____%2F04%3A00102843" target="_blank" >RIV/60077352:_____/04:00102843 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Statistical correlations of primer thermodynamic stability delta G0 for enhanced flax ISSR-PCR cultivar authentication

  • Original language description

    We revealed four statistically significant correlations related to inter-simple-sequence repeat :ISSR patterns: 1 between free energy G0 of ISSR primer and the reamplification intensity :dAi, 2 between free energy G0 of ISSR primer and PIC coefficient, 3resp. 4 between free energy G0 of anchor sequence of primer and the number of total, resp. polymorphic bands in ISSR patterns. Methodological recommendations for effective ISSR primer design were inferred based on revealed correlations.

  • Czech name

    Statistické korelace termodynamické stability PCR primerů ?G0 pro kvalitnější identifikaci kultivarů lnu užitím ISSR-PCR metody

  • Czech description

    Nalezli jsme čtyři statisticky významné korelace vztahující se ke gelovým spektrům ISSR-PCR: 1 mezi volnou energií ?G0 ISSR primeru a intenzitou reamplifikace PCR: dAi, 2 mezi volnou energií ?G0 ISSR primeru a PIC koeficientem, 3 resp. 4 mezi volnou energií ?G0 sekvenční kotvy primeru a počtem veškerých, resp. polymorfních pruhů v ISSR spektrech. Z těchto korelací byla vyvozena metodická doporučení pro efektivnější navrhování ISSR primerů.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA521%2F03%2F0019" target="_blank" >GA521/03/0019: Isolation of hypervariable microsatellites suitable for identification of flax germplasm.</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Agricultural and Food Chemistry

  • ISSN

    0021-8561

  • e-ISSN

  • Volume of the periodical

    52

  • Issue of the periodical within the volume

    -

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    4

  • Pages from-to

    2568-2571

  • UT code for WoS article

  • EID of the result in the Scopus database