Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077387%3A_____%2F03%3A62033016" target="_blank" >RIV/60077387:_____/03:62033016 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics.

Original language description

ELF97 phosphate is a phosphatase substrate which produces ELFA, a fluorescent water-insoluble product, upon hydrolysis. We studied the kinetics of ELFA precipitation in acidic Plešné Lake (pH about 5; high phosphatase activity) and eutrophic Římov reservoir (pH 7-10; moderate phosphatase activity). Relative fluorescence ELFA precipitates was quantified with image analysis. Time-courses of ELFA formation exhibited lag periods followed by finite periods of linear increase. In Plešné Lake, lag-times were 1-18 min and rates of increase in ELFA fluorescence higher (by about 2 orders of magnitude) than in Římov reservoir (lag-times 30-200 min). Linear regression of seasonal data on rates of increase in ELFA fluorescence from image cytometry and spectrofluorometry (r (2) = 0.65, n = 10) allowed for calibration of image cytometry. Preliminary measurements of extracellular phosphatase activities of several algae resulted in rates 10-2260 fmol cell(-1) h(-1).

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Environmental Microbiology

ISSN

1462-2912

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

462-472

UT code for WoS article

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EID of the result in the Scopus database

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