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Biolistic inoculation and improved detection of PVS by molecular hybridization

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F06%3A9P000919" target="_blank" >RIV/60109807:_____/06:9P000919 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Biolistic inoculation and improved detection of PVS by molecular hybridization

  • Original language description

    New extraction procedure was developed to detect potato virus Y and S by a dot-blot hybridization. Improved extraction buffer that contains 5% SDS enabled a sensitive detection without need to purify RNA. An alternative biolostic inoculation method was developed for potato virus S (PVS) which led to strong pathogenic effect on tomato.

  • Czech name

    Biolostická inokulace a zlepšená detekce PVS pomocí molekulární hybridizace

  • Czech description

    Byl vyvinut nový extrakční postup pro detekci PVY a PVS pomocí dot-blot hybridizace. Zlepšený extrakční pufr obsahující 5% SDS umožňoval citlivou detekci bez potřeby purifikovat RNA. Pro PVS byly vyvinuta alternativní metoda biolistické inokulace, pomocíkteré bylo dosahováno silného patogenního účinku na rajčatech.

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    GF - Diseases, pests, weeds and plant protection

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/QF3109" target="_blank" >QF3109: The differentiation of Potato Virus S (PVS) and the identification of isolates of quarantine significance by means of molecular diagnostic techniques.</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2006

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Sborník příspěvků XVII. česká a slovenská konference o ochraně rostlin

  • ISBN

    80-213-1564-4

  • ISSN

  • e-ISSN

  • Number of pages

    5

  • Pages from-to

  • Publisher name

    ÚVTI-Z

  • Place of publication

    Praha

  • Event location

    Praha

  • Event date

    Sep 12, 2006

  • Type of event by nationality

    CST - Celostátní akce

  • UT code for WoS article