A study of asymetric somatic hybridization in genus Solanum.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F07%3A8P001082" target="_blank" >RIV/60109807:_____/07:8P001082 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
A study of asymetric somatic hybridization in genus Solanum.
Original language description
Optimalization of protoplast organelles inactivation (nuclei, chloroplasts, mitochondria) with goals using them in cybridization is aim of this study. The effort is focused especially on use extranuclei inheritance of wild species to increase variabilityof cultivated potato. Inactivation of nuclei and semiautonomic organelles was studied in mesophyll protoplasts selected from the members of the genus Solanum tuberosum (cv. Kordoba, cv. Karin, cv. Komtesa) and Solanum verrucosum. Immediately after the isolation, an aliquot of protoplasts was UV-irradiated, these protoplasts did not regenerate. Inactivation of semiautonomic organelles was performed in aliquots of protoplasts with graded concentrations of IODOACETAMIDE (0.3 mM; 0.4 mM), RHODAMINE 6G (0.05 mM; 0.1 mM) and IODOACETIC ACID (0.125 mM; 0.2 mM). These concentrations of active substances did not damage protoplasts and the regeneration of a cell wall was sporadically recorded. In controls, division was observed and microcolonies
Czech name
Studium asymetrické somatické hybridizace u rodu Solanum.
Czech description
Byly optimalizovány podmínky inaktivace organel. Úsilí bylo zaměřeno na přenos mimojaderné dědičnosti Solanum verrucosum a zvýšení variability u kulturního bramboru S.tuberosum. Protoplasty byly ozářeny UV světlem nebo ošetřeny JODOACETAMID (0.3 mM; 0.4mM), RHODAMINE 6G (0.05 mM; 0.1 mM) a jodooctová kyselina(0.125 mM; 0.2 mM). Výsledkem bylo optimalizování koncentrace a doby působení. Ošetřené protoplasty byly fúzovány elektrickým polem nebo PEG. V asymetrických fúzích bylo dosaženo vyšších stupňů regenerace než u ošetřených kontrol.
Classification
Type
O - Miscellaneous
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/QF4108" target="_blank" >QF4108: Utilization of protoplast fusion technique in breeding of the important cultivated crops of the genera Brassica, Cucumis and Solanum.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů