Use of mitotic polyploidization and somatic hybridization for creating of new breeding materials in Solanum genus
Result description
Doubled materials were obtained using polyploidization (colchicine or oryzaline application to nodal segments) at wild speies S. berthaultii, S. bulbocastanum, S. pinnatisectum, S. verrucosum and dihaploids of Solanum tuberosum. Regenerants were evaluated by flow-cytometry. The derived tetraploids were used in inter and intra-specific crosses. Somatic hybridization was done by an electrofusion of mesophyll protoplasts. Solanum tuberosum (cv. Bintje, Ditta, Keřkovský rohlíček, Kordoba, Magda; dihaploid 243 and 299), S. berthaultii, S. bulbocastanum, S. pinnatisectum and S. verrucosum were used. Regenerated plants were assessed by flow-cytometry and a RAPD method. Somatic hybrids were obtained in several combinations.
Keywords
mitotic polyploidizationsomatic hybridizationSolanum tuberosumwild Solanum species
The result's identifiers
Result code in IS VaVaI
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Use of mitotic polyploidization and somatic hybridization for creating of new breeding materials in Solanum genus
Original language description
Doubled materials were obtained using polyploidization (colchicine or oryzaline application to nodal segments) at wild speies S. berthaultii, S. bulbocastanum, S. pinnatisectum, S. verrucosum and dihaploids of Solanum tuberosum. Regenerants were evaluated by flow-cytometry. The derived tetraploids were used in inter and intra-specific crosses. Somatic hybridization was done by an electrofusion of mesophyll protoplasts. Solanum tuberosum (cv. Bintje, Ditta, Keřkovský rohlíček, Kordoba, Magda; dihaploid 243 and 299), S. berthaultii, S. bulbocastanum, S. pinnatisectum and S. verrucosum were used. Regenerated plants were assessed by flow-cytometry and a RAPD method. Somatic hybrids were obtained in several combinations.
Czech name
Využití mitotické polyploidizace a somatické hybridizace pro tvorbu nových šlechtitelských materiálů u rodu Solanum
Czech description
Zdvojené materiály byly získány cestou polyploidizace (aplikací kolchicinu nebo oryzalinu na nodální segmenty) u planých druhů S. berthaultii, S. bulbocastanum, S. pinnatisectum, S. verrucosum a u dihaploidů Solanum tuberosum. Regeneranti byly hodnoceniprůtokovou cytometrií. Odvozené tetraploidy byly zařazeny do vnitro- a mezidruhových křížení. Somatická hybridizace byla uskutečněna elektrofúzí mezofylových protoplastů. Byly použity druhy Solanum tuberosum (cv. Bintje, Ditta, Keřkovský rohlíček, Kordoba, Magda; dihaploid 243 and 299), S. berthaultii, S. bulbocastanum, S. pinnatisectum a S. verrucosum. Regenerované rostliny byly hodnoceny průtokovou cytometrií a RAPD. Somatické hybridy byly získány u několika kombinací.
Classification
Type
O - Miscellaneous
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
QF4133: Development of advanced breeding materials with horizontal resistance genes to late blight.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Result type
O - Miscellaneous
CEP
EI - Biotechnology and bionics
Year of implementation
2007