Molecular probing of Potato Virus S Genome
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F19%3AN0000073" target="_blank" >RIV/60109807:_____/19:N0000073 - isvavai.cz</a>
Result on the web
<a href="https://www.vubhb.cz/cs/vedecke-prace-2019" target="_blank" >https://www.vubhb.cz/cs/vedecke-prace-2019</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Molecular probing of Potato Virus S Genome
Original language description
Complete genomes of three isolates of Potato virus S (PVS) were cloned and sequenced. The complete sequence of PVS genome enabled to develop an immunocapture RT-PCR probing of the PVS genome. Using this system, the sequence variability of 11 genome zones was examined for 34 PVS isolates including 15 PVS-CS variants that caused a systemic infection in Chenopodium quinoa. A broad variability between PVS isolates and diverse sequence variants was found. cDNA fragments covering the coat protein (CP) leader and CP-coding region (approx. 420 bp) were pooled for PVS-O and Chenopodium-systemic PVS isolates (PVS-CS) and corresponding cDNA libraries were screened for sequence variants. Genome probing suggested that the PVS-CS isolates analyzed were close to the ordinary European isolates of ordinary strain of PVS (PVS-O) but distant to the original Andean strain of PVS (PVS-A).
Czech name
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Czech description
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Classification
Type
J<sub>ost</sub> - Miscellaneous article in a specialist periodical
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Vědecké práce VÚB Havlíčkův Brod
ISSN
1802-940X
e-ISSN
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Volume of the periodical
25
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
10
Pages from-to
29-38
UT code for WoS article
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EID of the result in the Scopus database
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