Detection and relative quantification of amine oxidase gene (yobN) in Bacillus subtilis: application of real-time quantitative PCR
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG42__%2F21%3A00556954" target="_blank" >RIV/60162694:G42__/21:00556954 - isvavai.cz</a>
Alternative codes found
RIV/70883521:28110/22:63529198 RIV/70883521:28610/22:63529198
Result on the web
<a href="https://link.springer.com/article/10.1007/s13197-021-05090-9" target="_blank" >https://link.springer.com/article/10.1007/s13197-021-05090-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s13197-021-05090-9" target="_blank" >10.1007/s13197-021-05090-9</a>
Alternative languages
Result language
angličtina
Original language name
Detection and relative quantification of amine oxidase gene (yobN) in Bacillus subtilis: application of real-time quantitative PCR
Original language description
Degradation of undesirable biogenic amines (BAs) in foodstuffs by microorganisms is considered one of the most effective ways of eliminating their toxicity. In this study, we design two sets of primers for the detection and quantification of the amine oxidase gene (yobN) and endogenous (housekeeping) gene (gyrB) in Bacillus subtilis. Moreover, these sets can be used for relative quantification of yobN by real-time PCR (qPCR). We also tested the degradation of BAs by three bacterial strains (B. subtilis strains: IB1a, CCM 2216, CCM 2267) in a mineral medium over a two-day period. Their degradation abilities were verified by high performance liquid chromatography with UV detection (HPLC/UV). According to the results, two strains significantly (P < 0.05) reduced histamine, tyramine, putrescine, and cadaverine. Moreover, our results indicate that the degradation ability of B. subtilis strains could be limited by sporulation because the gene encoding amine oxidase (yobN) is no longer expressed in the spores.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21101 - Food and beverages
Result continuities
Project
<a href="/en/project/GA17-09594S" target="_blank" >GA17-09594S: Reduction of biogenic amines content in model systems</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
ISSN
0022-1155
e-ISSN
0975-8402
Volume of the periodical
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Issue of the periodical within the volume
58
Country of publishing house
IN - INDIA
Number of pages
8
Pages from-to
8
UT code for WoS article
000639073800002
EID of the result in the Scopus database
2-s2.0-85104147963