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Photometric microplate assay for estimation of paraoxon inhibited acetylcholinesterase reactivation efficacy

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F08%3A00001991" target="_blank" >RIV/60162694:G44__/08:00001991 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Photometric microplate assay for estimation of paraoxon inhibited acetylcholinesterase reactivation efficacy

  • Original language description

    Photometric microplate assay was performed for testing of paraoxon-inhibited acetylcholinesterase (AChE). Three reactivators were used for reactivation purposes: obidoxime, pralidoxime, and HI-6. 3-D graphs (percent of reactivation vs. concentration of reactivator and vs. time of reactivator effecting) were constructed for each reactivator to compare their efficacy. The best results were obtained when obidoxime was applied; reactivation was near to 80 %. Suitability of photometric microplates for following of reactivation procedures was mentioned in this work.

  • Czech name

    Fotometrické multikanálové stanovení účinnosti reaktivace acetylcholinesterázy inhibované paraoxonem

  • Czech description

    Multikanálová spektrofotometrie na titrační destičce byla použita pro testování reaktivace paraoxonem inhibované acetylcholinesterázy (AChE). Tři reaktivátory byly použity: obidoxim, pralidoxim a HI-6. 3-D grafy (procento reaktivace vs. koncentrace reaktivátoru vs. čas působení) byly konstruovány pro každý reaktivátor tak aby byla porovnána jejich účinnost. Nejlepší výsledky byly dosaženy s obidoximem (reaktivace 80%). Použitelnost prezentované metody je diskutována v práci.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    FP - Other medical fields

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Enzyme Inhibition and Medicinal Chemistry

  • ISSN

    1475-6366

  • e-ISSN

  • Volume of the periodical

    23

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    4

  • Pages from-to

  • UT code for WoS article

    000260847500005

  • EID of the result in the Scopus database