Bacterial protein complexes investigation using blue native PAGE

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F11%3A00002450" target="_blank" >RIV/60162694:G44__/11:00002450 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.micres.2010.01.005" target="_blank" >http://dx.doi.org/10.1016/j.micres.2010.01.005</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Bacterial protein complexes investigation using blue native PAGE

Original language description

Investigation of protein-protein interactions has arisen as a comprehensive approach for understanding bacterial physiology as well as pathogenesis. From this point of view, bacterial membrane as a place of contact with outer milieu seems to be crucial compartment and therefore, investigation of lipophilic proteins and their interactions is inevitable. Unfortunately, several methods developed for the analysis of protein interaction suffer from their labour intensiveness and underrepresent integral membrane proteins. Therefore, blue native polyacrylamide gel electrophoresis (BN-PAGE) with its simplicity and suitability for lipophilic entities has been widely employed in microbiological research. For investigation of membrane proteins interactions BN-PAGE became a method of choice. The efficacy in this area was proven by the elucidation not only of the stoichiometry, but also dynamic changes of several complexes involved in energetic metabolism, secretion and transport systems, localized

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microbiological Research

ISSN

0944-5013

e-ISSN

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Volume of the periodical

166

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

16

Pages from-to

47-62

UT code for WoS article

000285698700005

EID of the result in the Scopus database

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