A multiprotein binding interface in an intrinsically disordered region of the tumor suppressor protein interferon regulatory factor-1

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F11%3A00002567" target="_blank" >RIV/60162694:G44__/11:00002567 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/11:00371939

Result on the web

<a href="http://dx.doi.org/10.1074/jbc.M110.204602" target="_blank" >http://dx.doi.org/10.1074/jbc.M110.204602</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

A multiprotein binding interface in an intrinsically disordered region of the tumor suppressor protein interferon regulatory factor-1

Original language description

The interferon-regulated transcription factor and tumor suppressor protein IRF-1 is predicted to be largely disordered outside of the DNA-binding domain. One of the advantages of intrinsically disordered protein domains is thought to be their ability totake part in multiple, specific but low affinity protein interactions; however, relatively few IRF-1-interacting proteins have been described. The recent identification of a functional binding interface for the E3-ubiquitin ligase CHIP within the major disordered domain of IRF-1 led us to ask whether this region might be employed more widely by regulators of IRF-1 function. Here we describe the use of peptide aptamer-based affinity chromatography coupled with mass spectrometry to define a multiprotein binding interface on IRF-1 (Mf2 domain; amino acids 106-140) and to identify Mf2-binding proteins from A375 cells.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/LC07017" target="_blank" >LC07017: Oncoproteomics Centre</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biological Chemistry

ISSN

0021-9258

e-ISSN

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Volume of the periodical

286

Issue of the periodical within the volume

16

Country of publishing house

MX - MEXICO

Number of pages

14

Pages from-to

14291-14303

UT code for WoS article

000289556200052

EID of the result in the Scopus database

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