Cytokinesis-block micronucleus (CBMN) assay / CBMN cytome assay in human lymphocytes after in vitro irradiation and its use in biodosimetry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F11%3A00002616" target="_blank" >RIV/60162694:G44__/11:00002616 - isvavai.cz</a>
Result on the web
<a href="http://mmsl.pmfhk.cz/casopis/2011/mmsl_2011_1.pdf" target="_blank" >http://mmsl.pmfhk.cz/casopis/2011/mmsl_2011_1.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Cytokinesis-block micronucleus (CBMN) assay / CBMN cytome assay in human lymphocytes after in vitro irradiation and its use in biodosimetry
Original language description
Cytokinesis-block micronucleus (CBMN) assay and its comprehensive variant CBMN cytome assay are cytogenetic methods. CBMN is based on assessment of micronuclei in nucleated cells that have completed only one nuclear division. Besides micronuclei, CBMN cytome assay analyzes additional genotoxic (nucleoplasmic bridges and nuclear buds), cytostatic (nuclear division index), and cytotoxic (amount of necrotic and apoptotic cells) parameters. The aim of this study is to evaluate these parameters in human blood lymphocytes after in vitro irradiation and to assess its contribution to biodosimetry. Material and methods: Human blood from 6 donors was in vitro irradiated by 0, 1, 2, 3, 4, or 5 Gy and cultivated for 72 hours. Blood lymphocytes were stimulated withphytohemagglutinin and their cytokinesis was blocked by cytochalasin B. After cultivation, cultures were hypotonically treated, dropped onto glass slides and stained with Giemsa. Slides were evaluated by microscope. Results: We observed
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
<a href="/en/project/OVUOFVZ200812" target="_blank" >OVUOFVZ200812: Short-term and long-term nonspecific changes in organisms exposed to high and low doses of nerve agents on cellular and molecular level</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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