Evaluation of soman-induced extracranial histopathology in the context of clinical biochemistry, mitotic and apoptotic activity and morphometric analysis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F17%3A43875732" target="_blank" >RIV/60162694:G44__/17:43875732 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S1214021X16302435" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1214021X16302435</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jab.2016.09.005" target="_blank" >10.1016/j.jab.2016.09.005</a>

Result language

angličtina

Original language name

Evaluation of soman-induced extracranial histopathology in the context of clinical biochemistry, mitotic and apoptotic activity and morphometric analysis

Original language description

We aimed this study to evaluate histopathology of heart, lung, liver, kidney, small and large intestine in the context of clinical biochemistry, mitotic (intestine only) and apoptotic activity and morphometric analysis (intestine and lung only). Male Wistar rats were poisoned by soman (i.m., 52 mu g kg (1); 70% LD50). Samples were taken 1, 3 and 7 days following soman exposure. Biochemistry was evaluated in blood. Hematoxylin-eosin staining, Mallory&apos;s PTAH method, immunohistochemical detection of activated-caspase-3, TUNEL, and morphometric computer analysis were performed. We found increased AST and CK in blood, areas ranging from acute myolysis and necrosis to areas undergoing resolution in heart, a biphasic response consisting of hyperaemia, edema and bleeding leading to decreased airiness (day 1) and delayed inflammatory response with increased apoptosis (day 7) in lung, lymphangiectasis in small intestine (day 1) and subepithelial edema in large intestine (day 3) after soman intoxication. In intestine, mitotic activity decreased in crypts 1 and 7 days after the intoxication. Apoptotic activity decreased in the superficial compartment on the day 1, whereas it increased in the same compartment 3 days after the poisoning. Soman intoxication at the sublethal dose induced significant biochemical changes in blood and histopathological changes in heart, lung and intestine.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30108 - Toxicology

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Biomedicine

ISSN

1214-021X

e-ISSN

—

Volume of the periodical

15

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

23-31

UT code for WoS article

000396404200004

EID of the result in the Scopus database

2-s2.0-85001103507