Inter strand crosslinks in DNA induced in vivo by percutaneous application of sulphur mustard to rats and mice
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F18%3A43889569" target="_blank" >RIV/60162694:G44__/18:43889569 - isvavai.cz</a>
Alternative codes found
RIV/00179906:_____/18:10377868
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1383571818300822?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1383571818300822?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.mrgentox.2018.06.014" target="_blank" >10.1016/j.mrgentox.2018.06.014</a>
Alternative languages
Result language
angličtina
Original language name
Inter strand crosslinks in DNA induced in vivo by percutaneous application of sulphur mustard to rats and mice
Original language description
Inter-strand crosslinks (ICL) in the DNA are regarded to be the main toxic lesions induced by sulphur mustard (SM). We have followed the induction of ICL in the DNA of different organs of Wistar rats and Balb/c or NMRI mice by the percutaneous application of SM using the modified (reverse) comet assay. Significant amounts of ICL were found in Balb/C lymphocytes, in bone marrow and liver cells after the dose of 80 mg/kg. A dose-dependent amount of ICL was induced in rats, with efficient induction in lymphocytes and spleen cells already after 5 mg SM/kg, indicating a higher susceptibility of rats to the DNA-damaging effect of SM compared with mice. A significant induction of ICL in other tested tissues (liver, bone marrow, colon epithelium) was seen at the dose of 20 mg/kg. The induced ICL were removed from the DNA during 48 h except for rats at the dose of 80 mg/kg. In fact, we observed that ICL are almost completely repaired in tissues of rats receiving high lethal doses. Results suggest that the unhooking of ICL, which we followed with the comet assay, may lead to the formation of another toxic DNA lesion during the repair process.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30108 - Toxicology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Mutation Research-Genetic Toxicology and Environmental Mutagenesis
ISSN
1383-5718
e-ISSN
—
Volume of the periodical
832
Issue of the periodical within the volume
Aug
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
35-40
UT code for WoS article
000441855400005
EID of the result in the Scopus database
2-s2.0-85048761832