Natural immune booster imuregen significantly affects the proliferation of tumor cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F19%3A00555141" target="_blank" >RIV/60162694:G44__/19:00555141 - isvavai.cz</a>
Result on the web
<a href="https://www.mmsl.cz/pdfs/mms/2019/03/02.pdf" target="_blank" >https://www.mmsl.cz/pdfs/mms/2019/03/02.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.31482/mmsl.2019.007" target="_blank" >10.31482/mmsl.2019.007</a>
Alternative languages
Result language
angličtina
Original language name
Natural immune booster imuregen significantly affects the proliferation of tumor cells
Original language description
Background. Extracts from plant and/or animal tissues are frequently used in alternative medicine as drugs or food supplements. Such extracts may contain a complex of pharmacologically or physiologically active factors but frequently there exists no experimental confirmation as to precise mechanisms of action. This work aimed to verify if a long used bovine tissue extract Imuregen registered as a food supplement has desirable effect on tumor cells. Methods. Two independent methodological approaches were used. Viability of cell cultures was evaluated using WST-1-based cell cytotoxicity assay. Cell growth was monitored in real time using xCELLigence cell analysis. Normal human adherent lung fibroblasts (NHLF) were used to represent non - tumor lung cells. A human non-small cell lung carcinoma cell line H1299 was used as a model of tumor cells. Results. Our study demonstrated a direct influence on viability of the H1299 tumor cell line (p < 0.005) and a cytostatic/cytotoxic effect of the bovine tissue extract after 72h. of cultivation while leaving non-tumor NHLF cell line unaffected. The extract (0.1 µg/ml and 1 µg/ml, resp.) also significantly affected the viability of irradiated H1299 tumor cell line (p < 0.005, Co, 4Gy) compared to non-tumor irradiated counterparts. In addition to the cytotoxic effect, the extract slightly modified the generation time of the cells and substantial differences between the effects on tumor and non-tumor cell lines were observed. Conclusion. The data presented here might suggest the extract intervenes into the proliferative cell cycle and subsequently influences the generation time of cells. Further analyses should be oriented toward the effects of animal tissue extracts on cellular systems defending against tumors and/or infections and intercellular communications that lead to influencing the fate of individual cell types.
Czech name
—
Czech description
—
Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
—
OECD FORD branch
30102 - Immunology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Military Medical Science Letters
ISSN
0372-7025
e-ISSN
2571-113X
Volume of the periodical
88
Issue of the periodical within the volume
3
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
9
Pages from-to
106-114
UT code for WoS article
—
EID of the result in the Scopus database
2-s2.0-85073352799