Inhalation of molecular hydrogen prevents ischemia-reperfusion liver damage during major liver resection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F19%3A00555434" target="_blank" >RIV/60162694:G44__/19:00555434 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/19:10403617 RIV/00179906:_____/19:10403617

Result on the web

<a href="http://atm.amegroups.com/article/view/32526/html" target="_blank" >http://atm.amegroups.com/article/view/32526/html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.21037/atm.2019.11.43" target="_blank" >10.21037/atm.2019.11.43</a>

Result language

angličtina

Original language name

Inhalation of molecular hydrogen prevents ischemia-reperfusion liver damage during major liver resection

Original language description

Background: Liver resection is a surgical procedure associated with a high risk of hepatic failure that can be fatal. One of the key mechanisms involves ischemia-reperfusion damage. Building on the well-known positive effects of hydrogen at mitigating this damage, the goal of this work was to demonstrate the antioxidant, anti-inflammatory, and anti-apoptotic effects of inhaled hydrogen in domestic pigs during major liver resection. Methods: The study used a total of 12 domestic pigs, 6 animals underwent resection with inhaled hydrogen during general anesthesia, and 6 animals underwent the same procedure using conventional, unsupplemented, general anesthesia. Intraoperative preparation of the left branch of the hepatic portal vein and the left hepatic artery was performed, and a tourniquet was applied. Warm ischemia was induced for 120 minutes and then followed by liver reperfusion for another 120 minutes. Samples from the ischemic and non-ischemic halves of the liver were then removed for histological and biochemical examinations. Results: An evaluation of histological changes was based on a numerical expression of damage based on the Suzuki score. Liver samples in the group with inhaled hydrogen showed a statistically significant reduction in histological changes compared to the control group. Biochemical test scores showed no statistically significant difference in hepatic transaminases, alkaline phosphatase (ALP), lactate dehydrogenase (LD), and lactate. However, a surprising result was a statistically significant difference in gamma-glutamyl-transferase (GMT). Marker levels of oxidative damage varied noticeably in plasma samples. Conclusions: In this experimental study, we showed that inhaled hydrogen during major liver resection unquestionably reduced the level of oxidative stress associated with ischemia-reperfusion damage. We confirmed this phenomenon both histologically and by direct measurement of oxidative stress in the organism.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30204 - Oncology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Annals of Translational Medicine

ISSN

2305-5839

e-ISSN

2305-5847

Volume of the periodical

7

Issue of the periodical within the volume

23

Country of publishing house

CN - CHINA

Number of pages

12

Pages from-to

74

UT code for WoS article

000503257700063

EID of the result in the Scopus database

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