Piezoelectric Immunosensor for Tissue Transglutaminase Antibodies Determination for Celiac Disease Diagnostic and Comparison with ELISA Method
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F20%3A00555984" target="_blank" >RIV/60162694:G44__/20:00555984 - isvavai.cz</a>
Result on the web
<a href="http://www.electrochemsci.org/abstracts/vol15/150605154.pdf" target="_blank" >http://www.electrochemsci.org/abstracts/vol15/150605154.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.20964/2020.06.36" target="_blank" >10.20964/2020.06.36</a>
Alternative languages
Result language
angličtina
Original language name
Piezoelectric Immunosensor for Tissue Transglutaminase Antibodies Determination for Celiac Disease Diagnostic and Comparison with ELISA Method
Original language description
Celiac disease (CD) is a hereditary disease characterized by occurrence of IgA class antibodies against tissue transglutaminase (anti-tTG) in serum. In this work, we compared standard Enzyme-Linked Immunosorbent Assay (ELISA) with a quartz crystal microbalance (QCM) measuring system for determination of anti-tTG. The new immunosensor is based on immobilized tTG in an open conformation (open-tTG) and optimized for assay of IgA anti-tTG with limit of detection 13.3 RU/ml and good specificity and stability over time. Testing serum samples of patients suffering CD showed suitability of the prepared immunosensor for practical performance. Compare to ELISA requiring skilled personnel and expensive equipment, the newly constructed measuring system for measurement of QCM sensor is cheap, easy to prepare and does not require any special skill thus it can be introduced into any laboratory or general practitioner office.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
<a href="/en/project/TJ01000478" target="_blank" >TJ01000478: Construction of sensor for quantitative detection of antibodies against tissue transglutaminase for celiac disease diagnostics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Electrochemical Science
ISSN
1452-3981
e-ISSN
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Volume of the periodical
15
Issue of the periodical within the volume
6
Country of publishing house
RS - THE REPUBLIC OF SERBIA
Number of pages
12
Pages from-to
5154-5165
UT code for WoS article
000551697600027
EID of the result in the Scopus database
2-s2.0-85086838446