A colour sensor integrated into a microcontroller platform as a reliable tool for measuring pH changes in biochemistry applications
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F25%3A00563653" target="_blank" >RIV/60162694:G44__/25:00563653 - isvavai.cz</a>
Result on the web
<a href="https://pubs.rsc.org/en/content/articlelanding/2024/ay/d4ay00637b" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2024/ay/d4ay00637b</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/d4ay00637b" target="_blank" >10.1039/d4ay00637b</a>
Alternative languages
Result language
angličtina
Original language name
A colour sensor integrated into a microcontroller platform as a reliable tool for measuring pH changes in biochemistry applications
Original language description
Colorimetry is a widely used technique for optical detection in point-of-care testing and on-site detection. Although some studies employ a multiplex approach to analyse coloured solutions, many still analyse one sample at a time. We have prepared a simple and affordable colorimetric assay based on a TCS34725 colour sensor (ams-OSRAM) integrated into an M5Stack module and an RGB LED module both inserted into a 3D printed frame. We found that the colorimetric assay can be easily transferred to a colour sensing platform, and the signal range obtained using the prepared colorimeter is more than 200 times larger than that obtained using digital image colorimetry (DIC) for the same samples containing cholinesterase or urease as a model enzyme providing a change in pH of the processed solution. The assay appears to be ready for practical use. An open-source colorimeter with a colour sensor for determination of pH in liquids with a universal indicator - a novel approach for reaction monitoring.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Methods
ISSN
1759-9660
e-ISSN
1759-9679
Volume of the periodical
16
Issue of the periodical within the volume
38
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
6487-6493
UT code for WoS article
001304076300001
EID of the result in the Scopus database
2-s2.0-85203004328