Dexamethasone Acetate-Loaded PLGA Nanospheres Targeting Liver Macrophages
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F26%3A00564070" target="_blank" >RIV/60162694:G44__/26:00564070 - isvavai.cz</a>
Alternative codes found
RIV/62690094:18470/25:50021918 RIV/00216208:11150/25:10488107 RIV/00216208:11160/25:10488107
Result on the web
<a href="http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1616-5195" target="_blank" >http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1616-5195</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/mabi.202400411" target="_blank" >10.1002/mabi.202400411</a>
Alternative languages
Result language
angličtina
Original language name
Dexamethasone Acetate-Loaded PLGA Nanospheres Targeting Liver Macrophages
Original language description
Glucocorticoids are potent anti-inflammatory drugs, although their use is associated with severe side effects. Loading glucocorticoids into suitable nanocarriers can significantly reduce these undesirable effects. Macrophages play a crucial role in inflammation, making them strategic targets for glucocorticoid-loaded nanocarriers. The main objective of this study is to develop a glucocorticoid-loaded PLGA nanocarrier specifically targeting liver macrophages, thereby enabling the localized release of glucocorticoids at the site of inflammation. Dexamethasone acetate (DA)-loaded PLGA nanospheres designed for passive macrophage targeting are synthesized using the nanoprecipitation method. Two types of PLGA NSs in the size range of 100–300 nm are prepared, achieving a DA-loading efficiency of 19 %. Sustained DA release from nanospheres over 3 days is demonstrated. Flow cytometry analysis using murine bone marrow-derived macrophages demonstrates the efficient internalization of fluorescent dye-labeled PLGA nanospheres, particularly into pro-inflammatory macrophages. Significant down-regulation in pro-inflammatory cytokine genes mRNA is observed without apparent cytotoxicity after treatment with DA-loaded PLGA nanospheres. Subsequent experiments in mice confirm liver macrophage-specific nanospheres accumulation following intravenous administration using in vivo imaging, flow cytometry, and fluorescence microscopy. Taken together, the data show that the DA-loaded PLGA nanospheres are a promising drug-delivery system for the treatment of inflammatory liver diseases.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/EH22_008%2F0004607" target="_blank" >EH22_008/0004607: New Technologies for Translational Research in Pharmaceutical Sciences /NETPHARM</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Macromolecular Bioscience
ISSN
1616-5187
e-ISSN
1616-5195
Volume of the periodical
25
Issue of the periodical within the volume
2
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
2400411
UT code for WoS article
001366394400001
EID of the result in the Scopus database
2-s2.0-85210762746