Application of dicentric chromosome assay for evaluation of radioprotective effect
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F26%3A00564806" target="_blank" >RIV/60162694:G44__/26:00564806 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/biomethods/article/10/1/bpaf058/8222721?login=true" target="_blank" >https://academic.oup.com/biomethods/article/10/1/bpaf058/8222721?login=true</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/biomethods/bpaf058" target="_blank" >10.1093/biomethods/bpaf058</a>
Alternative languages
Result language
angličtina
Original language name
Application of dicentric chromosome assay for evaluation of radioprotective effect
Original language description
The dicentric chromosome assay is a well-established biodosimetric method used to assess absorbed ionizing radiation doses by detecting dicentric chromosomal aberrations. Here, we present a detailed, reproducible protocol for applying the dicentric chromosome assay for in vitro evaluation of radioprotective agents, including novel piperazine derivatives compared with amifostine and its active metabolite WR-1065. The protocol covers all key steps-blood sample preparation, in vitro irradiation, lymphocyte culture, metaphase preparation, and scoring of dicentric chromosomes. It highlights critical stages that affect data quality and reproducibility. Integrating manual scoring with automated analysis using the Metafer system ensures accurate and efficient assessment. Thus, this protocol bridges the fields of biological dosimetry and preclinical screening of radioprotective agents, providing a reliable framework for emergency radiation dose estimation and the development of new radiation medical countermeasures.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
<a href="/en/project/NU23-08-00256" target="_blank" >NU23-08-00256: Hit-to-lead development of small molecules for enhanced medical radiation protection</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biology Methods & Protocols
ISSN
—
e-ISSN
2396-8923
Volume of the periodical
10
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
bpaf058
UT code for WoS article
001548852200001
EID of the result in the Scopus database
2-s2.0-105014780638