Use of Controlled Exogenous Stress for Improvement of Poly(3-hydroxybutyrate) Production in Cupriavidus necator

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F10%3A%230000578" target="_blank" >RIV/60193697:_____/10:#0000578 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Use of Controlled Exogenous Stress for Improvement of Poly(3-hydroxybutyrate) Production in Cupriavidus necator

Original language description

The PHB production by Cupriavidus necator H16 depends on the type and concentration of stress factors and on the time of stress application. Hydrogen peroxide and ethanol significantly enhanced PHB accumulation in C. necator cells. Improved yields (10.9g/L PHB) were observed after exposure of bacterial culture to 0.5 mmol/L H2O2 at the beginning of cultivation and to additional peroxide stress (5 mmol/L H2O2) after 60 h of cultivation (beginning of the stationary phase). Production was then approximateto 28 % higher than in control (8.50 g/L PHB). The highest yields (11.2 g/L PHB) were observed when ethanol (0.5 %) was applied at the beginning of stationary phase. An application of exogenous stress could thus be used as a simple strategy for a significant improvement of PHB production in C. necator.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

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Continuities

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FOLIA MICROBIOLOGICA

ISSN

0015-5632

e-ISSN

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Volume of the periodical

55

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

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UT code for WoS article

000275992300003

EID of the result in the Scopus database

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