Determination of total carbohydrate content in beer using its pre-column enzymatic cleavage and HPLC-RI

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F14%3A%230000966" target="_blank" >RIV/60193697:_____/14:#0000966 - isvavai.cz</a>

Result on the web

<a href="http://link.springer.com/article/10.1007/s12161-014-9805-y" target="_blank" >http://link.springer.com/article/10.1007/s12161-014-9805-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12161-014-9805-y" target="_blank" >10.1007/s12161-014-9805-y</a>

Result language

angličtina

Original language name

Determination of total carbohydrate content in beer using its pre-column enzymatic cleavage and HPLC-RI

Original language description

Beside ethanol, carbohydrates are the main source of total energy in beer. While analyses of fermentable carbohydrates are important from the technological point of view, the total content of carbohydrates is relevant in terms of nutrition. A high-performance liquid chromatography (HPLC) method with refractometric (RI) detection was developed for determination of total carbohydrate content in beer. Using enzymatic reaction with amyloglucosidase, the carbohydrates were cleaved to yield glucose and shortglucose oligomers of less than 10 units, and separated on HPLC ion exchanger Rezex RSO-Oligosaccharide column in Ag+ mode. Optimum parameters were established for the enzymatic sample treatment and for the HPLC separation of reaction products. Calibration curves of glucose, fructose, maltose and simultaneously analyzed glycerol ranged from 0.001 to 0.5 g/100 ml, correlation coefficients of all calibration curves were 0.9999. The instrumental limits of quantification were 0.001 g/100 ml a

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Food Analytical Methods

ISSN

1936-9751

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

1677-1686

UT code for WoS article

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EID of the result in the Scopus database

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