Experimental adhesion of Geobacillus stearothermophilus and Anoxybacillus flavithermus to stainless steel compared with predictions from interaction models

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F20%3AN0000011" target="_blank" >RIV/60193697:_____/20:N0000011 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s11696-019-00880-0" target="_blank" >https://link.springer.com/article/10.1007/s11696-019-00880-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11696-019-00880-0" target="_blank" >10.1007/s11696-019-00880-0</a>

Result language

angličtina

Original language name

Experimental adhesion of Geobacillus stearothermophilus and Anoxybacillus flavithermus to stainless steel compared with predictions from interaction models

Original language description

Spore-forming thermophilic bacteria of the genus Geobacillus and Anoxybacillus are frequent contaminants in dairy industry. This study is the first attempt to apply models of physicochemical interactions (thermodynamic, DLVO, and XDLVO) to quantify their adhesion properties to stainless steel particles (SSP). The predictions of interaction models were compared with experimental data (contact angles, zeta potentials, size) regarding interacting surfaces (cells and SSP). Adhesion intensities (AI) were determined experimentally taking advantage of the magnetic properties of particulate stainless steel. The importance of weak physicochemical interactions was estimated by comparison of experimental AI with model predictions of colloidal interactions. The results revealed that the most reliable description of AI was obtained using the XDLVO model, including Lifshitz–van der Waals (LW), acid–base, and electrostatic (EL) interactions. The AI of cells to SSP at an ionic strength of 10 mM decreased in the order G. stearothermophilus DSM 456 > A. flavithermus DSM 2641 > G. stearothermophilus DSM 22, and the differences were statistically significant. At a higher ionic strength (100 mM), the highest AI was observed for A. flavithermus DSM 2641, but the differences between species studied were statistically insignificant. The main driving force for bacterial adhesion to SSP at 10 mM was EL interactions, while at 100 mM, the XDLVO model predicted favorable interactions between A. flavithermus DSM 2641 and SSP due to attractive LW forces.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemical Papers

ISSN

2585-7290

e-ISSN

1336-9075

Volume of the periodical

74

Issue of the periodical within the volume

1

Country of publishing house

CH - SWITZERLAND

Number of pages

8

Pages from-to

297-304

UT code for WoS article

000510432900023

EID of the result in the Scopus database

2-s2.0-85069510500