Laboratory scale cultivation of Salinispora tropica in shake flasks and mechanically stirred bioreactors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F21%3AN0000086" target="_blank" >RIV/60193697:_____/21:N0000086 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/21:43922743
Result on the web
<a href="https://link.springer.com/article/10.1007/s10529-021-03121-1" target="_blank" >https://link.springer.com/article/10.1007/s10529-021-03121-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10529-021-03121-1 " target="_blank" >10.1007/s10529-021-03121-1 </a>
Alternative languages
Result language
angličtina
Original language name
Laboratory scale cultivation of Salinispora tropica in shake flasks and mechanically stirred bioreactors
Original language description
Objective Marine actinomycetes from the genus Salinispora have an unexploited biotechnological potential. To accurately estimate their application potential however, data on their cultivation, including biomass growth kinetics, are needed but only incomplete information is currently available. Results This work provides some insight into the effect of temperature, salinity, nitrogen source, glucose concentration and oxygen supply on growth rate, biomass productivity and yield of Salinispora tropica CBN-440(T). The experiments were carried out in unbaffled shake flasks and agitated laboratory-scale bioreactors. The results show that the optimum growth temperature lies within the range 28-30 degrees C, salinity is close to sea water and the initial glucose concentration is around 10 g/L. Among tested nitrogen sources, yeast extract and soy peptone proved to be the most suitable. The change from unbaffled to baffled flasks increased the volumetric oxygen transfer coefficient (k(L)a) as did the use of agitated bioreactors. The highest specific growth rate (0.0986 h(-1)) and biomass productivity (1.11 g/L/day) were obtained at k(L)a = 28.3 h(-1). A further increase in k(L)a was achieved by increasing stirrer speed, but this led to a deterioration in kinetic parameters. Conclusions Improvement of S. tropica biomass growth kinetics of was achieved mainly by identifying the most suitable nitrogen sources and optimizing k(L)a in baffled flasks and agitated bioreactors.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BIOTECHNOLOGY LETTERS
ISSN
0141-5492
e-ISSN
1573-6776
Volume of the periodical
43
Issue of the periodical within the volume
9
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
1715-1722
UT code for WoS article
000651796800001
EID of the result in the Scopus database
2-s2.0-85106340490