Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F02%3A00004302" target="_blank" >RIV/60460709:41210/02:00004302 - isvavai.cz</a>

Alternative codes found

RIV/60460709:_____/02:00004302

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism

Original language description

Actual methods of molecular analyses of DNA are routinely used at fields of zoological and botanical taxonomy, pest management and plant breeding. Knowledge of species-composition in populations of potato cyst nematodes (Globodera rostochiensis, G. pallida) is very important for selection of appropriate measure of regulation PCN's occurrence. The molecular method for distinguishing of PCN species is described at this article. There were used European populations of PCN - Šluknov (Ro1), Obersteinbach (Ro2), Harmerz (Ro5), Kalle (Pa2), Chavornay (Pa3), Delmsen (Pa3), and some cysts of unknown patotype from Kašperské hory (K) locality. Species-specific sets of primers for ITS-1 (Internal Transcribed Spacer 1) amplification were designed on base of known sequences ITS-1 of both PCN species by using of freeware Primers! for the World Wide Web. By using of set Fro1 - Rro1 was product 411bp detected (only in cause G. rostochiensis), by using of set Fpa2 - Rpa1 the product 239bp was detected (

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GE - Plant cultivation

OECD FORD branch

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Project

<a href="/en/project/EP9107" target="_blank" >EP9107: Analysis of the Resistance Genes Donors against Potato Cyst Nematodes in Potato Genofond by DNA Markers</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Rostlinná výroba (Plant Production)

ISSN

0370-663X

e-ISSN

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Volume of the periodical

48

Issue of the periodical within the volume

11

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

4

Pages from-to

486-489

UT code for WoS article

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EID of the result in the Scopus database

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