Pokročilá analýza sekvencí 3 dánských Mop-top virů bramboru

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A8066" target="_blank" >RIV/60460709:41210/04:8066 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Pokročilá analýza sekvencí 3 dánských Mop-top virů bramboru

Original language description

The entire nucleotide sequence for the coding regions of a Danish PMTV isolate 54-15 was determined and compared to other known and sequenced isolates of PMTV. Many nucleotide and amino acid changes were found in parts of RNA coding for the triple gene block (TGB) proteins and part of the RNA coding for the read-through region of the coat protein (CP). These regions for two other isolates, the mild one 54-10 and the severe one 54-19, were sequenced. Only two amino acid changes were found that correlatedwith the subdivision of isolates according to symptom development into mild and severe subgroups. Although the sequence comparisons indicate a high genetic stability of PMTV populations, a surprising change was found in the newly sequenced isolates - the replacement of the AUG start codon of the fourth gene of the TGB encoding RNA, coding for a cystein-rich protein, by the less efficient GUG start codon.

Czech name

Extended sequence analysis of three Danish Potato mop-top virus (PMTV)

Czech description

The entire nucleotide sequence for the coding regions of a Danish PMTV isolate 54-15 was determined and compared to other known and sequenced isolates of PMTV. Many nucleotide and amino acid changes were found in parts of RNA coding for the triple gene block (TGB) proteins and part of the RNA coding for the read-through region of the coat protein (CP). These regions for two other isolates, the mild one 54-10 and the severe one 54-19, were sequenced. Only two amino acid changes were found that correlatedwith the subdivision of isolates according to symptom development into mild and severe subgroups. Although the sequence comparisons indicate a high genetic stability of PMTV populations, a surprising change was found in the newly sequenced isolates - the replacement of the AUG start codon of the fourth gene of the TGB encoding RNA, coding for a cystein-rich protein, by the less efficient GUG start codon.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Virus Genes

ISSN

0920-8569

e-ISSN

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Volume of the periodical

2

Issue of the periodical within the volume

29

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

249-255

UT code for WoS article

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EID of the result in the Scopus database

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