Polyclonal antibodies for detection of flour mite Acarus siro (Acari: Acaridida

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A11974" target="_blank" >RIV/60460709:41210/05:11974 - isvavai.cz</a>

Alternative codes found

RIV/00027006:_____/04:2807

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Polyclonal antibodies for detection of flour mite Acarus siro (Acari: Acaridida

Original language description

Acaroid mites are serious pests of stored products and households.They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need forrapid detection methods such as ELISA.The aim of our work was therefore to prepare and characterise antibodies for detection of the flour mite (Acarus siro). Two polyclonal antibodies (anti- A.siroK53 and anti-A.siroK54) were prepared against an antigenin the form of protein fraction from whole body homogenates of Acarus siro. Specificity and sensitivity of the prepared antibodies were evaluated.We did not observe any cross-reactivity with stored beetles, moths, other mite species, micro-fungi, the rearing diet of mites (yeast and wheat diet) and extracts from wheat kernels. Cross-reactivity to Acarus gracilis was found out. This phenomenon is discussed in relation to the evolution position of this species. The obtained antibodies (ant

Czech name

Polyklonální protilátky pro detekci roztoče moučného Acarus siro (Acari: Acaridida

Czech description

Acaroid mites are serious pests of stored products and households.They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need forrapid detection methods such as ELISA.The aim of our work was therefore to prepare and characterise antibodies for detection of the flour mite (Acarus siro). Two polyclonal antibodies (anti- A.siroK53 and anti-A.siroK54) were prepared against an antigenin the form of protein fraction from whole body homogenates of Acarus siro. Specificity and sensitivity of the prepared antibodies were evaluated.We did not observe any cross-reactivity with stored beetles, moths, other mite species, micro-fungi, the rearing diet of mites (yeast and wheat diet) and extracts from wheat kernels. Cross-reactivity to Acarus gracilis was found out. This phenomenon is discussed in relation to the evolution position of this species. The obtained antibodies (ant

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

<a href="/en/project/QF4071" target="_blank" >QF4071: Integrated method of detection and monitoring of pest contamination of stored-grain and cereal products by physical, chemical, immunochemical and molecular techniques.</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Phytophaga

ISSN

0393-8131

e-ISSN

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Volume of the periodical

2004

Issue of the periodical within the volume

14

Country of publishing house

IT - ITALY

Number of pages

10

Pages from-to

711-720

UT code for WoS article

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EID of the result in the Scopus database

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