Identification and evaluation of SCAR markers for molecular detection of the stem and bulb nematode Ditylenchus dipsaci.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F06%3A18210" target="_blank" >RIV/60460709:41210/06:18210 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Identification and evaluation of SCAR markers for molecular detection of the stem and bulb nematode Ditylenchus dipsaci.
Original language description
Ditylenchus dipsaci, the stem nematode, is a migratory endoparasite of over 500 species of angiosperms. The main method of control of D. dipsaci is crop rotation, but the presence of morphologically indistinguishable host races with different host preferences makes rotation generally ineffective. Therefore, a sensitive, rapid and reliable as well as cost effective technique is needed to identification of D. dipsaci in biological samples. This study describes the development of species-specific pairs ofPCR oligonucleotides for detection and identification of the stem nematode D. dipsaci in various plant hosts. Designed DIT-2 primer pair specifically amplified a fragment of 325 bp, while DIT-5 primer pair always produced a fragment of 245 bp in all D. dipsaci isolates. The resulting two developed SCAR primer pairs were further tested using as template DNA extracted from collection of twelve healthy plant hosts, but no amplification was observed. The PCR protocol was shown to be quite se
Czech name
Hledání a charkterizace SCAR markerů za účelem molekulární detekce osních nematod Ditylenchus dipsaci
Czech description
Ditylenchus dipsaci, the stem nematode, is a migratory endoparasite of over 500 species of angiosperms. The main method of control of D. dipsaci is crop rotation, but the presence of morphologically indistinguishable host races with different host preferences makes rotation generally ineffective. Therefore, a sensitive, rapid and reliable as well as cost effective technique is needed to identification of D. dipsaci in biological samples. This study describes the development of species-specific pairs ofPCR oligonucleotides for detection and identification of the stem nematode D. dipsaci in various plant hosts. Designed DIT-2 primer pair specifically amplified a fragment of 325 bp, while DIT-5 primer pair always produced a fragment of 245 bp in all D. dipsaci isolates. The resulting two developed SCAR primer pairs were further tested using as template DNA extracted from collection of twelve healthy plant hosts, but no amplification was observed. The PCR protocol was shown to be quite se
Classification
Type
D - Article in proceedings
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Proceedings of XVII. Czech and Slovak Plant Protection Conference. September 12-14, 2006,
ISBN
80-213-1564-4
ISSN
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e-ISSN
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Number of pages
10
Pages from-to
488-497
Publisher name
ČZU Praha
Place of publication
Praha
Event location
Praha
Event date
Sep 12, 2006
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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