Selective growth-inhibitory effect of 8-hydroxyquinoline towards Clostridium difficile and Bifidobacterium longum subsp. longum in co-culture analyzed by flow cytometry combined with fluorescent in situ hybridization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F14%3A66554" target="_blank" >RIV/60460709:41210/14:66554 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41340/14:66554

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Selective growth-inhibitory effect of 8-hydroxyquinoline towards Clostridium difficile and Bifidobacterium longum subsp. longum in co-culture analyzed by flow cytometry combined with fluorescent in situ hybridization

Original language description

The major risk factor for Clostridium difficile infection is the use of antibiotics owing to the disruption of the equilibrium of the host gut microbiota. To preserve the beneficial resident probiotic bacteria during infection treatment, the use of molecules with selective antibacterial activity enhances the efficacy by selectively removing C. difficile. One of them is the plant alkaloid 8-hydroxyquinoline (8HQ), which has been shown to selectively inhibit clostridia without repressing bifidobacteria. Selective antimicrobial activity is generally tested by culture techniques of individual bacterial strains. However, their main limitation is the inability to describe differential growth dynamics of more bacterial strains cultured together within the same experiment. In the present study we have combined fluorescent in situ hybridization and flow cytometry to describe the changes in active and non-active cells of a mixed culture formed by the opportunistic pathogen C. difficile CECT 531

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF MEDICAL MICROBIOLOGY

ISSN

0022-2615

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

63

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

1663-1669

UT code for WoS article

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EID of the result in the Scopus database

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