Assessment of genetic diversity of Czech sweet cherry cultivars using microsatellite markers

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F15%3A68869" target="_blank" >RIV/60460709:41210/15:68869 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.bse.2015.09.013" target="_blank" >http://dx.doi.org/10.1016/j.bse.2015.09.013</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bse.2015.09.013" target="_blank" >10.1016/j.bse.2015.09.013</a>

Result language
angličtina
Original language name
Assessment of genetic diversity of Czech sweet cherry cultivars using microsatellite markers
Original language description
We analyzed 24 sweet and wild cherry genotypes collected in Czech Republic to determine genetic variation, using previously described 16 SSR primers to adapt a fast, reliable method for preliminary screening and comparison of sweet cherry germplasm collections. All SSRs were polymorphic and they were able all together to distinguish unambiguously the genotypes. These SSR primers generated 70 alleles; the number of alleles per primer ranged from 2 to 7, with a mean of 4.4 putative alleles per primer combination. The primer UDP-98-412 gave the highest number of polymorphic bands (totally 7), while Empa2 and Empa3 gave the lowest number (2). The allele frequency varied from 2.1% to 87.5%. We observed 10% of unique alleles at different loci. The observed heterozygosity value ranged from 0.25 to 0.96 with an average of 0.72 while expected heterozygosity value varied from 0.22 to 0.75 with an average of 0.59. The PIC value ranged from 0.21 to 0.71 with a mean value of 0.523. Cluster analysis
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GE - Plant cultivation
OECD FORD branch
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Project
<a href="/en/project/QJ1210275" target="_blank" >QJ1210275: Solving of current problems of the sweat and sour cherry cultivation with commercial fruit quality focused on environmental favourable techniques</a><br>
Continuities
O - Projekt operacniho programu

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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