Physiological production of hydrogen sulfide is involved in porcine oocytes maturing in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F15%3A69195" target="_blank" >RIV/60460709:41210/15:69195 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.niox.2015.02.147" target="_blank" >http://dx.doi.org/10.1016/j.niox.2015.02.147</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.niox.2015.02.147" target="_blank" >10.1016/j.niox.2015.02.147</a>

Result language

čeština

Original language name

Physiological production of hydrogen sulfide is involved in porcine oocytes maturing in vitro

Original language description

Oocyte meiotic maturation is indispensable process of female gametogenesis. Hereby matured oocyte is predestined for fertilisation and subsequent embryonic development. The running of meiotic maturation is a result of activity of key factors – M-phase/Maturation Promoting Factor (MPF) and Mitogen Activated Protein Kinase(MAPK). Based on present knowledge, we hypothesise physiological action of hydrogen sulfide produced by CBS, CTH and 3-MPST, hydrogen sulfide releasing enzymes, during in vitro oocyte maturation.Oocytes were in vitro matured in modified M199 culture medium for 48 hrs in 39°C and 5% CO2. Consequently, parthenogenic activation of Na2S-treated oocytes by calcium ionophore A23185 was performed for evaluation of developmental competence. Our results demostrate the presence of mRNA CBS, CTH and 3-MPST CBS, CTH and 3-MPST themselves in porcine oocytes. The inhibition of present enzymes causes the deceleration of meiotic maturation and failure in achievement of fertilizing ability in a regula

Czech name

Physiological production of hydrogen sulfide is involved in porcine oocytes maturing in vitro

Czech description

Oocyte meiotic maturation is indispensable process of female gametogenesis. Hereby matured oocyte is predestined for fertilisation and subsequent embryonic development. The running of meiotic maturation is a result of activity of key factors – M-phase/Maturation Promoting Factor (MPF) and Mitogen Activated Protein Kinase(MAPK). Based on present knowledge, we hypothesise physiological action of hydrogen sulfide produced by CBS, CTH and 3-MPST, hydrogen sulfide releasing enzymes, during in vitro oocyte maturation.Oocytes were in vitro matured in modified M199 culture medium for 48 hrs in 39°C and 5% CO2. Consequently, parthenogenic activation of Na2S-treated oocytes by calcium ionophore A23185 was performed for evaluation of developmental competence. Our results demostrate the presence of mRNA CBS, CTH and 3-MPST CBS, CTH and 3-MPST themselves in porcine oocytes. The inhibition of present enzymes causes the deceleration of meiotic maturation and failure in achievement of fertilizing ability in a regula

Type

D - Article in proceedings

CEP classification

GG - Zootechnics

OECD FORD branch

—

Project

<a href="/en/project/QJ1510138" target="_blank" >QJ1510138: Innovation of reproductive biotechiques in farm animals</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

NITRIC OXIDE-BIOLOGY AND CHEMISTRY

ISBN

—

ISSN

1089-8603

e-ISSN

—

Number of pages

2

Pages from-to

59-60

Publisher name

ACADEMIC PRESS INC ELSEVIER SCIENCE

Place of publication

SAN DIEGO, USA

Event location

Athens, GREECE

Event date

May 3, 2015

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000353313900138