Quantitative detection of four pome fruit viruses in apple trees throughout the year
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F16%3A70898" target="_blank" >RIV/60460709:41210/16:70898 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.14601/Phytopathol_Mediterr-16825" target="_blank" >http://dx.doi.org/10.14601/Phytopathol_Mediterr-16825</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.14601/Phytopathol_Mediterr-16825" target="_blank" >10.14601/Phytopathol_Mediterr-16825</a>
Alternative languages
Result language
angličtina
Original language name
Quantitative detection of four pome fruit viruses in apple trees throughout the year
Original language description
A one-step real-time RT-PCR assay (RT-qPCR) with melting curve analysis, using the green fluorescence dye SYBR Green I, was developed to detect and quantify RNA targets from Apple mosaic virus (ApMV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV) and Apple chlorotic leaf spot virus (ACLSV) in infected apple trees. Single PCR products of 87 bp (ApMV), 70 bp (ASGV), 104 bp (ASPV) and 148 bp (ACLSV) were obtained, and melting curve analyses revealed distinct melting temperature peaks for each virus. A dilution series using in vitro synthesized transcripts containing the target sequences as standards yielded a reproducible quantitative assay, with a wide dynamic range of detection and low coefficients of variance. The content of selected viruses in apple plant tissues was stable throughout the year, and their accumulation did not significantly change between different plant tissues. The only minor exceptions were for ApMV and ACLSV, in which noticeable differences in their concentrati
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PHYTOPATHOLOGIA MEDITERRANEA
ISSN
0031-9465
e-ISSN
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Volume of the periodical
55
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
18
Pages from-to
207-224
UT code for WoS article
000381767900005
EID of the result in the Scopus database
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