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Quantitative detection of four pome fruit viruses in apple trees throughout the year

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F16%3A70898" target="_blank" >RIV/60460709:41210/16:70898 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.14601/Phytopathol_Mediterr-16825" target="_blank" >http://dx.doi.org/10.14601/Phytopathol_Mediterr-16825</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.14601/Phytopathol_Mediterr-16825" target="_blank" >10.14601/Phytopathol_Mediterr-16825</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Quantitative detection of four pome fruit viruses in apple trees throughout the year

  • Original language description

    A one-step real-time RT-PCR assay (RT-qPCR) with melting curve analysis, using the green fluorescence dye SYBR Green I, was developed to detect and quantify RNA targets from Apple mosaic virus (ApMV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV) and Apple chlorotic leaf spot virus (ACLSV) in infected apple trees. Single PCR products of 87 bp (ApMV), 70 bp (ASGV), 104 bp (ASPV) and 148 bp (ACLSV) were obtained, and melting curve analyses revealed distinct melting temperature peaks for each virus. A dilution series using in vitro synthesized transcripts containing the target sequences as standards yielded a reproducible quantitative assay, with a wide dynamic range of detection and low coefficients of variance. The content of selected viruses in apple plant tissues was stable throughout the year, and their accumulation did not significantly change between different plant tissues. The only minor exceptions were for ApMV and ACLSV, in which noticeable differences in their concentrati

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GF - Diseases, pests, weeds and plant protection

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    PHYTOPATHOLOGIA MEDITERRANEA

  • ISSN

    0031-9465

  • e-ISSN

  • Volume of the periodical

    55

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    18

  • Pages from-to

    207-224

  • UT code for WoS article

    000381767900005

  • EID of the result in the Scopus database