Semen freezability of endangered Old Kladruber horse – a pilot study

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F16%3AN0000012" target="_blank" >RIV/60460709:41210/16:N0000012 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Semen freezability of endangered Old Kladruber horse – a pilot study
Original language description
The aim of this study was to evaluate sperm characteristics of Old Kladruber stallions cryopreserved in two packaging systems. Each stallion was collected three times and samples were pre diluted and centrifuged (650 x g/15 min). Afterwards, Gent extender (MiniTube, Germany) with 20 percent (v/v) of egg yolk was added to obtain a final concentration at least 250 x 106 of progressively motile spermatozoa/ID. Extended semen was loaded either into 0,5 ml plastic straws or into 5 ml aluminum tubes. Horizontal freezing was performed in a styrofoam box (Animal Reproduction systems, Chino CA, USA). Motility was measured using CASA (Nis Elements, ver. 4,30, Laboratory Imaging, Prague, Czech Republic). The following kinematic parameters of sperm motility were evaluated: curvilinear velocity (VCL, um/s), straight line velocity (VSL, um/s) and average path velocity (VAP, um/s). Viability was evaluated using carboxyfluorescein diacetate and propidium iodide. The incidence of apoptotic sperms was evaluated with Yo Pro1 staining using propidium iodide and DAPI. Ten insemination doses from each stallion (5x 0,5 ml straws and 5x 5 ml aluminum tubes) were evaluated after thawing.
Czech name
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Czech description
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Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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