New Insight into Sperm Capacitation: A Novel Mechanism of 17ß-Estradiol Signalling
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A77579" target="_blank" >RIV/60460709:41210/18:77579 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.3390/ijms19124011" target="_blank" >http://dx.doi.org/10.3390/ijms19124011</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms19124011" target="_blank" >10.3390/ijms19124011</a>
Alternative languages
Result language
angličtina
Original language name
New Insight into Sperm Capacitation: A Novel Mechanism of 17ß-Estradiol Signalling
Original language description
Estradiol is a natural estrogen regulating reproduction including sperm and egg development, sperm maturation, called capacitation, and sperm egg communication. High doses can increase germ cell apoptosis and decrease sperm count. Our aim was to answer the biological relevance of estradiol in sperm capacitation and its effect on motility and acrosome reaction to quantify its interaction with estrogen receptors and propose a model of estradiol action during capacitation using kinetic analysis. Estradiol increased protein tyrosine phosphorylation, elevated rate of spontaneous acrosome reaction, and altered motility parameters measured HamiltonThorne Computer Assisted Semen Analyzer (CASA) in capacitating sperm. To monitor time and concentration dependent binding dynamics of extracellular estradiol, high-performance liquid chromatography with tandem mass spectrometry was used to measure sperm response and data was subjected to kinetic analysis. The kinetic model of estradiol action during sperm maturati
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
1422-0067
Volume of the periodical
19
Issue of the periodical within the volume
12
Country of publishing house
CH - SWITZERLAND
Number of pages
19
Pages from-to
1-19
UT code for WoS article
000455323500313
EID of the result in the Scopus database
2-s2.0-85058764880