Cysteine peptidases of Eudiplozoon nipponicum: a broad repertoire of structurally assorted cathepsins L in contrast to the scarcity of cathepsins B in an invasive species of haematophagous monogenean of common carp
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A78039" target="_blank" >RIV/60460709:41210/18:78039 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/18:00489775 RIV/00216224:14310/18:00100859 RIV/00216208:11310/18:10376237
Result on the web
<a href="http://dx.doi.org/10.1186/s13071-018-2666-2" target="_blank" >http://dx.doi.org/10.1186/s13071-018-2666-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13071-018-2666-2" target="_blank" >10.1186/s13071-018-2666-2</a>
Alternative languages
Result language
angličtina
Original language name
Cysteine peptidases of Eudiplozoon nipponicum: a broad repertoire of structurally assorted cathepsins L in contrast to the scarcity of cathepsins B in an invasive species of haematophagous monogenean of common carp
Original language description
Transcriptomic analysis revealed a set of ten distinct transcripts that encode EnCLs. The enzymes are significantly variable in their active sites, specifically the S2 subsites responsible for interaction with substrates. Some of them display unusual structural features that resemble cathepsins B and S. Two recombinant EnCLs had different pH activity profiles against both synthetic and macromolecular substrates, and were able to hydrolyse blood proteins and collagen I. They were localised in the haematin cells of the worm digestive tract and in gut lumen. The EnCB showed similarity with cathepsin B2 of Schistosoma mansoni. It displays molecular features typical of cathepsins B, including an occluding loop responsible for its exopeptidase activity. Although the EnCB hydrolysed haemoglobin in vitro, it was localised in the vitelline cells of the parasite and not the digestive tract.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30310 - Parasitology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Parasites & Vectors
ISSN
1756-3305
e-ISSN
1756-3305
Volume of the periodical
11
Issue of the periodical within the volume
142
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
17
Pages from-to
1-17
UT code for WoS article
000427131600001
EID of the result in the Scopus database
2-s2.0-85043226328