Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR–RFLP of kdr mutation in sodium channel gene

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F19%3A80432" target="_blank" >RIV/60460709:41210/19:80432 - isvavai.cz</a>
Alternative codes found
RIV/62968335:_____/19:N0000003 RIV/00216224:14310/19:00112657 RIV/00027006:_____/19:00005093
Result on the web
<a href="https://link.springer.com/article/10.1007%2Fs10493-019-00353-9" target="_blank" >https://link.springer.com/article/10.1007%2Fs10493-019-00353-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10493-019-00353-9" target="_blank" >10.1007/s10493-019-00353-9</a>

Result language
angličtina
Original language name
Detection of tau-fluvalinate resistance in the mite Varroa destructor based on the comparison of vial test and PCR–RFLP of kdr mutation in sodium channel gene
Original language description
Varroa destructor is the major cause of honey bee (Apis mellifera) colony losses. Mite control is limited to several miticides. The overuse of tau-fluvalinate has resulted in resistance via a knockdown resistance (kdr) mutation in the sodium channel gene NaVChs (L925V I M). In this study, we used the discriminating concentration of tau-fluvalinate (0,25 microg mL) to detect the resistance of mites in a bioassay. Further, we verified the presence of the kdr mutation in mites from the bioassay via PCR amplification of a fragment of the voltage-gated sodium channel gene (NaVCh), restriction fragment length polymorphisms (RFLPs), and densitometry analyses in pools of surviving or dead mites. Resistance values corresponding to the densitometry of the resistant allele were related to mite survival. In the vial test, the survival of the control group was significantly higher (70,4percent) than that of the tau-fluvalinate-treated group (34,3 percent). Mite survival in the vial test was significantly correlat
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/QJ1530148" target="_blank" >QJ1530148: Management of Varroa destructor resistance to acaricides improving honey bee (Apis mellifera) health.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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