Oxygen consumption rate of Caenorhabditis elegans as a highthroughput endpoint of toxicity testing using the Seahorse XFe 96 Extracellular Flux Analyzer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F20%3A83692" target="_blank" >RIV/60460709:41210/20:83692 - isvavai.cz</a>

Result on the web

<a href="https://www.nature.com/articles/s41598-020-61054-7" target="_blank" >https://www.nature.com/articles/s41598-020-61054-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-020-61054-7" target="_blank" >10.1038/s41598-020-61054-7</a>

Result language

angličtina

Original language name

Oxygen consumption rate of Caenorhabditis elegans as a highthroughput endpoint of toxicity testing using the Seahorse XFe 96 Extracellular Flux Analyzer

Original language description

Caenorhabditis elegans presents functioning, biologically relevant phenotypes and is frequently used as a bioindicator of toxicity. However, most C. elegans in vivo efect-assessment methods are laborious and time consuming. Therefore, we developed a novel method to measure the oxygen consumption rate of C. elegans as a sublethal endpoint of toxicity. This protocol was tested by exposing 50 larval stage one C. elegans individuals for 48h (at 20C) to diferent concentrations of two toxicants i.e. benzylcetyldimethylammonium chloride (BAC-C16) and cadmium (Cd). Following exposures, the oxygen consumption rate of the C. elegans individuals were measured using the high-throughput functionality of the Seahorse XFe 96 Extracellular Flux Analyzer. Furthermore, a strong, positive correlation was evidenced between C. elegans oxygen consumption rate and a commonly used, ecologically relevant endpoint of toxicity (growth inhibition) for BAC-C16 and Cd. The data presented in this study show that C. elegans oxygen

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10511 - Environmental sciences (social aspects to be 5.7)

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Volume of the periodical

10

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

1-11

UT code for WoS article

000560102000007

EID of the result in the Scopus database

2-s2.0-85081389929